LOCUS       40924_23759       12000 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB722 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12000)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12000)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2033..2053)
                     /label=attB2
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             2065..2718
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             2725..2742
                     /codon_start=1
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
                     /translation="LVPRGS"
     terminator      2764..3016
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3050..3066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3074..3090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3098..3128)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3143..3164)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3200..3452)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3490..4785)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61223.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3490..4785)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4837..5016)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5522..5546)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6067..6855
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7104..7692
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7878..8018)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8362..8556)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8625..9695)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10127..10753)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatgtcc cctatactag gttattggaa aattaagggc
     2101 cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg
     2161 tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt
     2221 cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata
     2281 cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt
     2341 tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt
     2401 aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg
     2461 ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac
     2521 ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg
     2581 ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac
     2641 ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt
     2701 ggcgaccatc ctccaaaatc ggatctggtt ccgcgtggat ccgcttagag ctcgaatttc
     2761 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     2821 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     2881 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     2941 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     3001 tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg tcatagctgt
     3061 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3121 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga attaggcgcg
     3181 cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     3241 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     3301 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     3361 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     3421 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgcctcgag tgacttctct
     3481 gattgcagac tatttatacc aaccagcaag aaagtgtcga agtatgaagc agaaggcgca
     3541 ggctagagcc tggaaaacga gaagatggat gcaaagagac ttttctgatt taggaccaaa
     3601 caacctcaag taaacattga cgagtgtccc atcttttgtg ccggtcagta atccggtagc
     3661 aggatcatac cttgggagac gatggcgcgg acatttcaca atcccagcaa gctgaggaag
     3721 cgataataga aggttcagaa cttgaggaag aaagaagatg aggagggttt cactgaagtg
     3781 acccagaata ccaacaactg ccatagtcat tccggcgaag actgtgtaag tgtctccaac
     3841 aaaaactgaa gaaggatacc aattgtaagc aagcattgcc aaggatgtgg ccatcagcgg
     3901 ttgagtaaga aatatcgaga aggcatgagc ttggtgatac tcgggatcaa cagatgctcc
     3961 gatttgcata acgttgtgta tcaggatagc agcggcaata acaactgttt gaccgatttc
     4021 aaggccattc agaccagcgt ggatgttaat agagtttgta caaaagacag caagtagccc
     4081 catatataac ttatatatcc ttcctagatt aagcacttcc aagccaatat aagcaacaag
     4141 aggttttggt ataacaatag ttgtatgacc agcataagcc atcaaaagtg gaagggtagc
     4201 aaaagatggc aggacaagtt ttactctcca aggcacatca aggacatcat ctacaaatcc
     4261 aagcaaaatc atgaagcaaa tagatgctag tgctgcatta tactccacaa gccaattaga
     4321 atcttcagtg aagttaaaat actgaaatat tatcgccacg atcaaaaaga caataccaac
     4381 aacaataccc aatgactctg gcactttaat atctccttga ggcgtgcctc ttttgttgat
     4441 atcaaaacca aacatgttac gtctcagaac gtatctggca gtaacaggaa tcattttgag
     4501 agtgacgaaa aacccaacca agctaagccc cgcgttaatt agaatcgatc gcttgagttc
     4561 attatcaacc ttgtagtgaa aacagagaag gtatagatag agcgaacaga gaagagtaga
     4621 gatcacgaag atcactccta atttcggcgg cgcgagacga aattcctcac cggaagcaga
     4681 aaccgtcttt cgggtcatct tctgctccga tggcgctgag ttgggttccg acgggtccgg
     4741 tttgttggga attgagatcg atgaagctct cttacgagct gccatatctt attgcccccc
     4801 gggatcagat ctggattgag agtgaatatg agactctaat tggataccga ggggaattta
     4861 tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc
     4921 gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa
     4981 acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     5041 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct
     5101 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     5161 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     5221 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     5281 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     5341 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     5401 cttgcggcag cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg
     5461 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     5521 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     5581 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     5641 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     5701 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     5761 acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg
     5821 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     5881 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     5941 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     6001 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     6061 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     6121 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     6181 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     6241 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     6301 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     6361 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     6421 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     6481 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     6541 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     6601 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     6661 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     6721 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     6781 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     6841 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     6901 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     6961 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     7021 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7801 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7861 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7921 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     7981 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     8041 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8101 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     8161 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     8221 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     8281 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     8341 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     8401 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     8461 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     8521 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     8581 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     8641 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     8701 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     8761 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     8821 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     8881 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     8941 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     9001 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     9061 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     9121 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     9181 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     9241 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     9301 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
     9361 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
     9421 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
     9481 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
     9541 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
     9601 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
     9661 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
     9721 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
     9781 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
     9841 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
     9901 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
     9961 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    10021 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    10081 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    10141 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    10201 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    10261 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    10321 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    10381 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    10441 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    10501 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    10561 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    10621 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    10681 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    10741 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    10801 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    10861 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    10921 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    10981 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    11041 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    11101 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    11161 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    11221 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    11281 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    11341 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    11401 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    11461 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    11521 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    11581 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    11641 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    11701 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    11761 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    11821 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    11881 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    11941 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
